論文種別	原著(症例報告除く)
言語種別	英語
査読の有無	査読あり
表題	Sequential PET estimation of cerebral oxygen metabolism with spontaneous respiration of O-15-gas in mice with bilateral common carotid artery stenosis
掲載誌名	正式名：JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM ISSNコード：0271-678X/1559-7016
出版社	SAGE PUBLICATIONS INC
巻・号・頁	37(10),pp.3334-3343
著者・共著者	Takashi Temma,Makoto Yamazaki,Jun Miyanohara,Hisashi Shirakawa,Naoya Kondo,Kazuhiro Koshino,Shuji Kaneko,Hidehiro Iida
発行年月	2017/10
概要	Positron emission tomography with O-15-labeled gases (O-15-PET) is important for invivo measurement of cerebral oxygen metabolism both in clinical and basic settings. However, there are currently no reports concerning O-15-PET in mice. Here, we developed an O-15-PET method applicable to mice with spontaneous respiration of O-15-gas without a tracheotomy catheter. Sequential O-15-PET was also performed in a mouse model of chronic cerebral hypoperfusion with bilateral common carotid artery stenosis (BCAS) induced by placement of microcoils. O-15-gas with isoflurane was supplied to the nose of mouse with evacuation of excess O-15-gas surrounding the body. O-15-PET was performed on days 3, 7, 14, 21, and 28 after surgery. Cerebral blood flow (CBF), cerebral blood volume, oxygen extraction fraction (OEF), and cerebral metabolic rate of oxygen (CMRO2) were calculated in whole brains. A significant decrease in CBF and compensatory increase in OEF in the BCAS group produced CMRO2 values comparable to that of the sham group at three days post-operation. Although CBF and OEF in the BCAS group gradually recovered over the first 28 days, the CMRO2 showed a gradual decrease to 68% of sham values at 28 days post-operation. In conclusion, we successfully developed a noninvasive O-15-PET method for mice.
DOI	10.1177/0271678X17692815
PMID	28178896