論文種別	原著(症例報告除く)
言語種別	英語
査読の有無	その他(不明)
表題	Elucidating the Light-Emitting Species Generated from Aminoluciferin in Firefly/Beetle Bioluminescence.
掲載誌名	正式名：Chemical & pharmaceutical bulletin 略　 称：Chem Pharm Bull (Tokyo) ISSNコード：13475223/00092363
掲載区分	国外
巻・号・頁	73(8),pp.678-686
著者・共著者	Hideo Takakura, Ryosuke Kojima, Takeaki Ozawa, Yasuteru Urano
発行年月	2025/08
概要	Oxyluciferin is the key photon emitter in the firefly/beetle luciferase-catalyzed bioluminescence reaction, and elucidating its chemical form within the active site of luciferase is essential for understanding and modulating emission wavelength. In this study, we focused on aminoluciferin (AL), a d-luciferin (d-LH2) analog in which the hydroxy group is substituted with an amino group. AL has been widely used as an alternative substrate to d-LH2 and in the development of bioluminescence probes. To identify the emissive form of AL in bioluminescence, we synthesized OxyAL (the emitter derived from AL) and 5-methyl-OxyAL, and measured their fluorescence spectra under various conditions. We assigned the range of emission wavelengths of keto-, enol- and enolate-OxyAL using 5,5-dimethyl-OxyAL, fixed in the keto form, as a reference. Based on spectral assignments and comparison with the bioluminescence spectra of AL, we conclude that enolate-OxyAL is the most probable light-emitting species in the active site of luciferase. These findings provide new insight into AL-based bioluminescence.
DOI	10.1248/cpb.c25-00302
PMID	40790510