論文種別	原著(症例報告除く)
言語種別	英語
査読の有無	その他(不明)
表題	Shotgun Label-Free Proteomic Analysis for Identification of a Potential Diagnostic Biomarker for Pancreatic Cancer.
掲載誌名	正式名：Biomedicines 略　 称：Biomedicines ISSNコード：22279059/22279059
掲載区分	国外
巻・号・頁	13(11),pp.2631
著者・共著者	Tetsushi Yamamoto, Shogen Boku, Kuniko Mitamura, Atsushi Taga
発行年月	2025/10
概要	Background: Pancreatic cancer (PC) is one of the cancers with poor prognosis. Although carbohydrate antigen (CA) 19-9 is currently the most specific and sensitive biomarker for the diagnosis of PC and, therefore, the most frequently used, approximately 34% of PC patients may have a Lewis antigen-negative phenotype and secrete little, if any, CA19-9. Therefore, effective alternative diagnostic methods for PC is required. Methods: In this study, we examined whether differentially expressed proteins in the blood of PC patients could be identified by global shotgun proteomics analysis using an in vitro cell line sample. Results: We identified 142 candidate proteins with differential expression in PC cells. A semiquantitative method and bioinformatic analysis led to a total of 14 candidate proteins that could potentially be detected in blood. Validation studies revealed that the expression of fibulin-1 was lower in PC cells than in normal pancreatic ductal cells. Moreover, in vivo fibulin-1 expression was significantly lower in serum from PC patients than in healthy individuals. Conclusions: These findings suggest that lower serum levels of fibulin-1 may be a novel biomarker for the detection of PC.
DOI	10.3390/biomedicines13112631
PMID	41301725