論文種別	原著(症例報告除く)
言語種別	英語
査読の有無	その他(不明)
表題	Differences in microRNA expression in rat liver following single- and combined administration of caffeine and dextromethorphan
掲載誌名	正式名：Fundamental toxicological sciences 略　 称：Fundam Toxicol Sci ISSNコード：2189115X
掲載区分	国内
出版社	一般社団法人 日本毒性学会
巻・号・頁	12(5),pp.155-163
著者・共著者	Emoto Yuko, Matsumoto Tomohiro, Hashiyada Masaki, Obayashi Masahiro, Yoshizawa Katsuhiko, Akane Atsushi
発行年月	2025
概要	<p>In forensic practice, blood drug levels are important determinants in the diagnosis of deaths related to drug poisoning. Even at non-lethal concentrations, potential drug interactions must be considered when multiple drugs are detected. MicroRNAs (miRNAs) have attracted increasing attention owing to their short length, robustness, and resistance to postmortem changes. Caffeine and dextromethorphan are among the most commonly abused substances by young people in Japan. However, reports on interactions between caffeine and dextromethorphan and comprehensive miRNA analyses following the administration of these drugs are limited. We aimed to determine whether miRNA expression differs in rats administered caffeine and dextromethorphan alone or in combination. We performed a comprehensive analysis of miRNAs in the livers of rats treated with caffeine, dextromethorphan, or both drugs. Eight miRNAs showed changes in expression exclusively in the combination treatment group, with five upregulated and three downregulated. Among these, rno-miR-34a-5p expression was significantly decreased in the combination group, as confirmed by quantitative RT-PCR. Although no differences were observed between groups in behavioral and pathological assessments at the administered doses, distinct changes in miRNA expression were detected. These results suggest that miRNAs may serve as biomarkers to indicate pathological differences between single and combined drug administration.</p>
DOI	10.2131/fts.12.155
NAID	1390306200188314496