論文種別	原著(症例報告除く)
言語種別	英語
査読の有無	その他(不明)
表題	Molecular tension indicators reveal unexpectedly complex regulation of tension in live mouse organs.
掲載誌名	正式名：Communications biology 略　 称：Commun Biol ISSNコード：23993642/23993642
巻・号・頁	pp.Online ahead of print.
著者・共著者	Keita Fujiwara, Katsunori Fujiki, Tomoya O Akama, Katsuhiko Shirahige, Ichiro Shiojima, Tomoyuki Nakamura, Maretoshi Hirai
発行年月	2026/02
概要	Since the emergence of molecular tension sensors, the understanding of mechanical forces has advanced substantially. However, visualizing molecular tension in mammalian tissues has remained challenging owing to the technical constraints of the Förster resonance energy transfer (FRET)-based molecular tension sensors. Here, we develop a molecular tension sensor based on circularly permuted EGFP with an elastic linker, which is inserted into either αActinin or αCatenin and then fused with mCherry at the C-terminus to simultaneously visualize tension and the amount of sensor protein. This single-fluorophore tension indicator enables subtle tension changes to be visualized simply from color tone under superresolution microscopy. We further generate H11 knock-in mice expressing these indicators, revealing a molecular-specific regulation of mechanical load within tissues. Thus, our molecular tension indicators provide a powerful approach for probing the complex and heterogeneous regulation of mechanical forces in vivo mammalian systems.
DOI	10.1038/s42003-026-09746-0
PMID	41714452