論文種別	原著(症例報告除く)
言語種別	英語
査読の有無	査読あり
表題	Intrinsic period stability of the cyanobacterial circadian oscillator across in vitro and in vivo conditions.
掲載誌名	正式名：Proceedings of the National Academy of Sciences of the United States of America 略　 称：Proc Natl Acad Sci U S A ISSNコード：10916490/00278424
掲載区分	国外
巻・号・頁	123(12),pp.e2526714123
著者・共著者	Kumiko Ito-Miwa, Keiko Imai, Kazuki Terauchi, Takao Kondo
発行年月	2026/03
概要	A defining feature of circadian clocks that enables adaptation to Earth's rotation is their ability to sustain an approximately 24 h period with precision, regardless of environmental factors such as temperature. This remarkable reliability of circadian timing can be reconstituted in vitro using only three cyanobacterial clock proteins: KaiA, KaiB, and KaiC. In vivo circadian rhythms, however, are governed not only by this Kai protein-based oscillator but also by transcription-translation feedback loops and additional clock components. The contribution of the Kai protein-based circadian oscillator to the overall reliability of the in vivo circadian rhythm remains unclear. In this study, we compared over 20 KaiC period mutants with periods ranging from 15 to 60 h under in vitro and in vivo conditions. In both cases, the period was insensitive to environmental conditions, suggesting a compensatory mechanism independent of metabolic state or rhythm amplitude. The ATPase activity of KaiC, the pacemaker of the cyanobacterial circadian clock, exhibited a stronger correlation with in vitro circadian frequency than in vivo circadian frequency. These results indicate that the KaiC ATPase-driven protein-based circadian oscillator inherently encodes a reliable circadian period independent of rhythm amplitude or environmental conditions. This intrinsic property likely plays a critical role in preserving the precision and stability of circadian timing in vivo while being influenced by the intracellular environment.
DOI	10.1073/pnas.2526714123
PMID	41849375