| 論文種別 | 原著(症例報告除く) |
| 言語種別 | 英語 |
| 査読の有無 | その他(不明) |
| 表題 | Generation of functional mesothelial cells from human iPSCs that restore peritoneal integrity in experimental peritoneal injury. |
| 掲載誌名 | 正式名：Stem cell research & therapy 略 称：Stem Cell Res Ther ISSNコード：17576512/17576512 |
| 掲載区分 | 国外 |
| 巻・号・頁 | pp.Online ahead of print |
| 著者・共著者 | Tadashi Kato, Mayu Yamashita, Masahiro Yasuda, Yutaro Ando, Ryusuke Nakatsuka, Yasumasa Shirouzu, Tatsuya Fujioka, Masayuki Tsukasaki, Fumiyuki Hattori, Yoshihiro Taniyama, Hiroaki Ogata, Akiko Sakashita, Hirokazu Honda, Hirofumi Hitomi |
| 発行年月 | 2026/04 |
| 概要 | BACKGROUND:Long-term peritoneal injury results in loss of mesothelial integrity and impaired ultrafiltration, limiting the durability of peritoneal dialysis. Because primary human peritoneal mesothelial cells (PMCs) are difficult to obtain and expand, regenerative strategies for restoring peritoneal function have remained limited.METHODS:We developed a protocol to differentiate human induced pluripotent stem cells (iPSCs) into functional peritoneal mesothelial-like cells (iPMCs). The molecular and functional characteristics of iPMCs were evaluated using gene expression analyses and functional assays, including comparison with a mesothelial cell model. The therapeutic effect of iPMCs was evaluated in a methylglyoxal-induced experimental peritoneal injury mouse model.RESULTS:iPMCs exhibited typical cobblestone morphology and expressed key mesothelial markers including CK18, WT1 and MSLN. Transcriptomic analysis demonstrated strong similarity between iPMCs and primary PMCs. In vivo, intraperitoneal administration of iPMCs partially improved peritoneal transport parameters in a mouse model of peritoneal injury.CONCLUSIONS:We successfully generated functional mesothelial cells from human iPSCs that reproduce mesothelial-like functional properties and suggest potential for restoring peritoneal integrity. This study demonstrates the feasibility of iPSC-derived mesothelial cells as a regenerative cell source for peritoneal repair and provides a foundation for future peritoneal regeneration therapy. |
| DOI | 10.1186/s13287-026-05037-x |
| PMID | 42035221 |