論文種別	原著(症例報告除く)
言語種別	英語
査読の有無	その他(不明)
表題	TMPRSS2-induced Golgi disruption restricts the incorporation of virus envelope glycoproteins into virions.
掲載誌名	正式名：EMBO reports 略　 称：EMBO Rep ISSNコード：14693178/1469221X
掲載区分	国外
巻・号・頁	27(12),pp.3424-3453
著者・共著者	Sayuri Seki, Shigeyoshi Harada, Akiko Sugimoto-Ishige, Midori Unno, Machie Sakuma, Shinsuke Ito, Hiroyuki Yamamoto, Aki Tanabe, Saori Matsuoka, Chieko Makino-Okamura, Sewon Ki, Hidehiro Fukuyama, Michishige Harada, Kazuya Tsumagari, Koshi Imami, Takashi Saito, Masato Kubo, Tadaki Suzuki, Haruhiko Koseki, Tetsuro Matano, Kosuke Miyauchi
発行年月	2026/06
概要	Understanding the relationship between viral proteins and host factors is essential for developing strategies to control virus infections. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infects host cells using angiotensin converting enzyme 2 (ACE2) and transmembrane protease serine 2 (TMPRSS2) as viral receptor and priming protease during the viral entry phase. Here, we report that TMPRSS2 reduces the infectivity of SARS-CoV-2 and HIV-1 during the viral production phase. Treatment of virus-producing cells with a TMPRSS2 inhibitor increases the production of infectious virions. TMPRSS2 enzymatic activity specifically disrupts the trans-Golgi by phosphorylating Golgi stacking proteins through ERK activation, which disturbs virion spike incorporation and structural maturation of the viral envelope glycoproteins, causing lower viral infectivity. We find that SARS-CoV-2 envelope protein (E protein) counteracts this TMPRSS2 activity to rescue SARS-CoV-2-S incorporation. These results demonstrate negative regulation of viral envelope glycoprotein incorporation by TMPRSS2 and reveal that SARS-CoV-2 regulates the Golgi system to create an optimal viral replication environment.
DOI	10.1038/s44319-026-00797-2
PMID	42156913