論文種別	原著(症例報告除く)
言語種別	英語
査読の有無	査読あり
表題	Estimating animal species from blood using in-gel digestion peptide mass fingerprinting.
掲載誌名	正式名：Analytical methods : advancing methods and applications 略　 称：Anal Methods ISSNコード：17599679/17599660
掲載区分	国外
巻・号・頁	18(32),pp.6929-6937
著者・共著者	Akihisa Igoh, Atsushi Akane
発行年月	2026/08
概要	Increasing numbers of domestic and wild animals in residential environments have contributed to a rise in animal-related incidents. As a result, the forensic identification of animal species from trace blood evidence has become increasingly important. In our previously reported simplified protocol for hemoglobin peptide mass fingerprinting (PMF), applicability was limited to mammals and required comparatively large blood volumes. To address these constraints, we implemented an in-gel digestion PMF workflow and sought to determine the optimal protein content for analysis. Pooled blood from 10 animals was subjected to SDS-PAGE, followed by Mascot-based protein identification. Subsequently, to evaluate species applicability and the suitability of the method for bloodstain analysis, in-gel digestion PMF was performed on blood samples from 21 species and bloodstains from 10 species at the optimized protein load. In nearly all specimens, hemoglobin derived from the target or phylogenetically related species yielded the highest protein scores. Overall, in-gel digestion PMF enables species estimation across a broad range of forensic animal samples while requiring a small blood volume. Given that PMF can be conducted using a relatively low-cost single mass spectrometer, this approach has the potential to advance the adoption of forensic proteomics.
DOI	10.1039/d6ay01360k
PMID	42478446