| 論文種別 | 原著(症例報告除く) |
| 言語種別 | 英語 |
| 査読の有無 | その他(不明) |
| 表題 | Immunohistochemical Characterization of Keratan Sulfate Preferentially Expressed in Papillary Thyroid Carcinoma Using a Novel Monoclonal Antibody 299-1C1. |
| 掲載誌名 | 正式名：The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 略 称：J Histochem Cytochem ISSNコード：15515044/00221554 |
| 掲載区分 | 国外 |
| 巻・号・頁 | pp.Online ahead of print |
| 著者・共著者 | Eiichi Kato, Tomoya O Akama, Natsumi Yonemoto, Akifumi Muramoto, Shigeharu Fujieda, Motohiro Kobayashi |
| 発行年月 | 2026/08 |
| 概要 | Keratan sulfate (KS) is a glycosaminoglycan consisting of repeating N-acetyllactosamine disaccharides, in which both galactose and N-acetylglucosamine are often 6-O-sulfated. It has been reported that KS recognized by the monoclonal antibodies 5D4 and 373E1 is preferentially expressed in papillary thyroid carcinoma (PTC) and only minimally in other thyroid tumors/lesions or normal thyroid tissue. However, the precise epitopes recognized by these antibodies remain incompletely characterized, and the expression and extent of low-sulfated KS in PTC have not been systematically evaluated. To better understand the nature of KS expressed in PTC, we generated a novel anti-KS monoclonal antibody, 299-1C1, and performed immunohistochemical analyses using 299-1C1 together with two existing anti-KS monoclonal antibodies, 5D4 and R-10G, in combination with keratanase II and endo-β-galactosidase. The results showed that both highly sulfated and low-sulfated KS are preferentially expressed in PTC, including lymph node metastases, accompanied by upregulation of genes encoding key KS biosynthetic enzymes (B3GNT7, B4GALT4, CHST2, and CHST6) in integrated TCGA/GTEx transcriptomic datasets. Expression of B3GNT7, B4GALT4, and CHST2, but not CHST6, was further increased in BRAF-mutant PTCs. These findings indicate that anti-KS monoclonal antibodies are useful for the pathological diagnosis of PTC, particularly for distinguishing lymph node metastases from intranodal thyroid inclusions. |
| DOI | 10.1369/00221554261479793 |
| PMID | 42665361 |